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rabbit polyclonal anti p2 receptor primary antibody  (Alomone Labs)


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    Alomone Labs rabbit polyclonal anti p2 receptor primary antibody
    Rabbit Polyclonal Anti P2 Receptor Primary Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+primary+antibody+for+p2y12/Anti-P2Y12+Receptor+(extracellular)+Antibody/pmc04360032-166-28-76
    Average 93 stars, based on 19 article reviews
    rabbit polyclonal anti p2 receptor primary antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Incubation:

    Article Title: P2Y12 Receptor Upregulation in Activated Microglia Is a Gateway of p38 Signaling and Neuropathic Pain
    Article Snippet: .. Membranes were incubated with Blocking One P (Nakarai, Kyoto, Japan) in Tris buffer containing Tween 20 (TBS-T) (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 0.2% Tween 20) for at least 20 min at room temperature and incubated with the polyclonal primary antibody for P2Y12 (1:200; Alomone Labs, Jerusalem, Israel), p38 (1:400; Cell Signaling Technology), p-p38 (1:400; Cell Signaling Technology), and -actin (1:2000; Sigma) at 4°C overnight. .. Membranes were then washed twice with TBST and probed with goat anti-rabbit IgG conjugated with horseradish peroxidase (1:2000; Chemicon) at room temperature for 2 h. Membranes were finally washed several times with TBST to remove unbound secondary antibodies and visualized by chemiluminescence using CSPD readyto-use reagent (Roche, Indianapolis, IN).

    Blocking Assay:

    Article Title: P2Y12 Receptor Upregulation in Activated Microglia Is a Gateway of p38 Signaling and Neuropathic Pain
    Article Snippet: .. Membranes were incubated with Blocking One P (Nakarai, Kyoto, Japan) in Tris buffer containing Tween 20 (TBS-T) (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 0.2% Tween 20) for at least 20 min at room temperature and incubated with the polyclonal primary antibody for P2Y12 (1:200; Alomone Labs, Jerusalem, Israel), p38 (1:400; Cell Signaling Technology), p-p38 (1:400; Cell Signaling Technology), and -actin (1:2000; Sigma) at 4°C overnight. .. Membranes were then washed twice with TBST and probed with goat anti-rabbit IgG conjugated with horseradish peroxidase (1:2000; Chemicon) at room temperature for 2 h. Membranes were finally washed several times with TBST to remove unbound secondary antibodies and visualized by chemiluminescence using CSPD readyto-use reagent (Roche, Indianapolis, IN).



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    P2X4, P2X7 and <t>P2Y12</t> immunoexpression in AMC in postnatal rat brain . By immunofluorescence labeling, P2X4 is colocalized with OX42 in the amoeboid microglial cells. Colocalization of OX42 and P2X4 in cells (arrows) is seen in the corpus callosum and subventricular zone (Aa-c, Scale bar = 100 μm). Immunopositive cells are round and have a typical morphology of amoeboid microglial cells (B/Ca-c. Scale bar = 20 μm). The amoeboid microglial cells exhibit a stronger P2X4 immunoreactivity compared with that of P2X7 and P2Y12 (D/Ea-c, Scale bar = 20 μm.).
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    polyclonal primary antibody for p2y12  (Alomone Labs)
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    P2X4, P2X7 and <t>P2Y12</t> immunoexpression in AMC in postnatal rat brain . By immunofluorescence labeling, P2X4 is colocalized with OX42 in the amoeboid microglial cells. Colocalization of OX42 and P2X4 in cells (arrows) is seen in the corpus callosum and subventricular zone (Aa-c, Scale bar = 100 μm). Immunopositive cells are round and have a typical morphology of amoeboid microglial cells (B/Ca-c. Scale bar = 20 μm). The amoeboid microglial cells exhibit a stronger P2X4 immunoreactivity compared with that of P2X7 and P2Y12 (D/Ea-c, Scale bar = 20 μm.).
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    Image Search Results


    P2X4, P2X7 and P2Y12 immunoexpression in AMC in postnatal rat brain . By immunofluorescence labeling, P2X4 is colocalized with OX42 in the amoeboid microglial cells. Colocalization of OX42 and P2X4 in cells (arrows) is seen in the corpus callosum and subventricular zone (Aa-c, Scale bar = 100 μm). Immunopositive cells are round and have a typical morphology of amoeboid microglial cells (B/Ca-c. Scale bar = 20 μm). The amoeboid microglial cells exhibit a stronger P2X4 immunoreactivity compared with that of P2X7 and P2Y12 (D/Ea-c, Scale bar = 20 μm.).

    Journal: BMC Neuroscience

    Article Title: Hypoxia induced amoeboid microglial cell activation in postnatal rat brain is mediated by ATP receptor P2X4

    doi: 10.1186/1471-2202-12-111

    Figure Lengend Snippet: P2X4, P2X7 and P2Y12 immunoexpression in AMC in postnatal rat brain . By immunofluorescence labeling, P2X4 is colocalized with OX42 in the amoeboid microglial cells. Colocalization of OX42 and P2X4 in cells (arrows) is seen in the corpus callosum and subventricular zone (Aa-c, Scale bar = 100 μm). Immunopositive cells are round and have a typical morphology of amoeboid microglial cells (B/Ca-c. Scale bar = 20 μm). The amoeboid microglial cells exhibit a stronger P2X4 immunoreactivity compared with that of P2X7 and P2Y12 (D/Ea-c, Scale bar = 20 μm.).

    Article Snippet: The sections were then incubated at 22-24°C with rabbit anti-P2X4 polyclonal primary antibody (1: 200, Alomone, Cat: APR-002, Yiselie), rabbit anti-P2X7 polyclonal primary antibody (1: 200, Alomone, Cat: APR-004, Yiselie), and rabbit anti-P2Y12 polyclonal primary antibody (1: 200, Alomone, Cat: APR-012, Yiselie) overnight.

    Techniques: Immunofluorescence, Labeling